Protective role of remote ischemic per-conditioning in acute renal injury induced by ischemia reperfusion via TLR-4 and TNF-α signaling pathway in rats

Authors

Abstract:

sIntroduction: Acute kidney injury (AKI) induced by ischemia-reperfusion (I / R) of the kidney as an inflammatory process in which multiple inflammatory factors are involved. Recently, one of the modalities of inflammation in AKI is Remote Ischemic Per-Conditioning (RIPerC). Materials and Methods: In this study, bilateral renal artery and vein occlusion were done for 45 minute and reperfusion and 24 h. The RIPerC model consisted of four five-minute cycles (two minutes of left femoral artery occlusion and three minutes of reperfusion) performed at the onset of renal ischemia. Rats were divided into control, I / R and RIPerC groups. At the end of the reperfusion period, urine, blood and kidney tissue samples were collected for functional, structural and molecular analysis. Results: Ischemia-reperfusion caused tissue damage and consequently renal dysfunction, which was shown by decreasing creatinine clearance and increasing relative excretion of sodium. In addition, in the I / R group, TLR-4 and TNF-α in mRNA levels were increased, whereas RIPerC simultaneously with I / R improved kidney structure and function and decreased TLR-4 and TNF-α expression levels. Conclusion: RIPerC protected the kidney against ischemia-reperfusion-induced kidney injury and probably this protective TEMPeffect was exerted by inhibition of TLR-4 signaling pathway in the kidney.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

The role of nitric oxide in the protective action of remote ischemic per-conditioning against ischemia/reperfusion-induced acute renal failure in rat

Objective(s): We investigated the role of nitric oxide (NO) in the protective effects of remote ischemic per-conditioning (rIPerC) on renal ischemia/reperfusion (I/R) injury in male rats. Materials and Methods: I/R treatment consisted of 45 min bilateral renal artery ischemia and 24 hr reperfusion interval. rIPerC was performed using four cycles of 2 min occlusions of the left femoral artery an...

full text

Protective effect of remote ischemic per-conditioning in the ischemia and reperfusion-induce renal injury in rats.

OBJECTIVE To evaluate the protective effect of remote ischemic per-conditioning in ischemia and reperfusion-induced renal injury. METHODS Fifteen rats (Rattus norvegicus) were randomized into three groups (n = 5): Group Normality (GN), Control Ischemia and Reperfusion (GIR) and Group remote ischemic per-conditioning (GPER). With the exception of the GN group, all others underwent renal ischem...

full text

Protective effects of dexmedetomidine and remote ischemic preconditioning on renal ischemia reperfusion injury in rats.

BACKGROUND The aim of this study was to evaluate the effects of remote ischemic preconditioning (RIPC) and dexmedetomidine as pharmacological conditioning in a rat renal ischemia/reperfusion (IR) injury model. METHODS Total of 28 male Wistar Albino rats weighing 250 to 300 g were divided into 4 equal groups. Group I (Sham; n=7): Laparotomy and renal pedicle dissection were performed, and the ...

full text

Protective Effect of Crocin on Gastric Mucosal Lesions Induced by Ischemia-Reperfusion Injury in Rats

The present study aimed to evaluate the protective effect of crocin on gastric mucosal lesions caused by ischemia-reperfusion (I/R) injury in rats.Forty male rats were randomly divided into sham, control (I/R injury) and three crocin-pretreated groups. To induce I/R lesions, the celiac artery was clamped for 30 min and then the clamp was removed to allow reperfusion for 3 h. Pretreated-rats rec...

full text

Protective Effect of Crocin on Gastric Mucosal Lesions Induced by Ischemia-Reperfusion Injury in Rats

The present study aimed to evaluate the protective effect of crocin on gastric mucosal lesions caused by ischemia-reperfusion (I/R) injury in rats.Forty male rats were randomly divided into sham, control (I/R injury) and three crocin-pretreated groups. To induce I/R lesions, the celiac artery was clamped for 30 min and then the clamp was removed to allow reperfusion for 3 h. Pretreated-rats rec...

full text

Renoprotective effects of GABA on ischemia/reperfusion- induced renal injury in hyperglycemic male and female rats

Introduction: Acute kidney injury (AKI) has been known as a complex clinical complication in diabetic patients. The main cause of AKI is ischemia/reperfusion injury (IRI). This study was designed to investigate the protective effects of GABA on renal IRI in hyperglycemic female and male rats. Methods: Sixty STZ induced diabetic male and female Wistar rats were categorized in 10 groups (5 fem...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 25  issue 1

pages  0- 0

publication date 2020-02

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

No Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023